Molecular Biotechnology – Recombinant DNA Technology


Content, progress and pedagogy of the module

Learning objectives


Students who complete the module must be able to

  • account for general technologies in molecular biology and genomic analyses
  • account for basic methods in recombinant DNA technology (cloning, vectors, primer design and transformation)
  • account for methods in genetics, epigenetics, transcriptomics and proteomics
  • account for basic techniques in DNA sequencing (genomes and fragments)
  • explain the basic methods for microbiome assessment and identification
  • explain the methods used in protein engineering (tagging, isolation, prediction and analyses)
  • explain the theoretical background of these methods, their advantages and limitations as well as possible applications


  • use molecular tools for heterologous expression of genes and proteins
  • perform DNA alignments of genes or fragments
  • optimize and design primers for PCR amplification of target DNA
  • determine variation in microbiome in environmental matrices
  • detect and isolate heterologously produced proteins

Type of instruction

  • Lectures
  • Workshops
  • Experimental exercises

Extent and expected workload

150 hours



Name of examMolecular Biotechnology – Recombinant DNA Technology
Type of exam
Written or oral exam
Assessment7-point grading scale
Type of gradingInternal examination
Criteria of assessmentThe criteria of assessment are stated in the Examination Policies and Procedures

Facts about the module

Danish titleMolekylær bioteknologi - rekombinant DNA-teknologi
Module codeK-KT-K1-31
Module typeCourse
Duration1 semester
Language of instructionEnglish
Empty-place SchemeYes
Location of the lectureCampus Esbjerg
Responsible for the module


Education ownerMaster of Science (MSc) in Engineering (Bioengineering)
Study BoardStudy Board of Chemistry and Bioscience
DepartmentDepartment of Chemistry and Bioscience
FacultyThe Faculty of Engineering and Science