Development of Recombinant Biocatalysts


Recommended prerequisite for participation in the module

The module builds on knowledge gained in Biomolecules and Biochemistry I, Biochemistry II, Microbiological processes or similar

Content, progress and pedagogy of the module

In the project, a selected biocatalyst is designed and developed 

The work includes design and construction of plasmids using different molecular techniques (e.g restriction enzyme based cloning and/or PCR based cloning), selection of relevant selection markers, transformation of E. coli, selection of correct E. coli clones, plasmid preparation and transformation of relevant host (if another host than E. coli is chosen) and selection and use of assays for testing and evaluation of the heterologous expressed gene(s) 

The project report should include a theoretical part and an experimental part with results and conclusion of the laboratory work

Learning objectives


Students who have passed the module 

  • Can describe the different steps in development of a selected biocatalysis with respect to cloning and transformation technologies as well as specific assays for testing the recombinant biocatalysts
  • Has knowledge on molecular biological techniques and basic bioinformatic tools


  • Hands on experience with common good laboratory practices (GLP) for working with recombinant microorganisms
  • Hands on experience with sterile techniques
  • Experience with basic microbial and molecular biological methods 


  • Can design a molecular project
  • Is able to work independently in a laboratory environment
  • Can reflect upon and develop own learning processes

Type of instruction

  • Project work

Extent and expected workload

450 hours



Name of examDevelopment of Recombinant Biocatalysts
Type of exam
Oral exam based on a project
Assessment7-point grading scale
Type of gradingExternal examination
Criteria of assessmentThe criteria of assessment are stated in the Examination Policies and Procedures

Facts about the module

Danish titleUdvikling af rekombinante biokatalysatorer
Module codeK-BBT-B5-15
Module typeProject
Duration1 semester
Language of instructionEnglish
Empty-place SchemeYes
Location of the lectureCampus Copenhagen
Responsible for the module
Time allocation for external examinersB


Education ownerBachelor of Science (BSc) in Engineering (Sustainable Biotechnology)
Study BoardStudy Board of Chemistry and Bioscience
DepartmentDepartment of Chemistry and Bioscience
FacultyThe Faculty of Engineering and Science