Content, progress and pedagogy of the module

Learning objectives

Knowledge

Students who complete the module must be able to

• account for general technologies in molecular biology and genomic analyses
• account for basic methods in recombinant DNA technology (cloning, vectors, primer design and transformation)
• account for methods in genetics, epigenetics, transcriptomics and proteomics
• account for basic techniques in DNA sequencing (genomes and fragments)
• explain the basic methods for microbiome assessment and identification
• explain the methods used in protein engineering (tagging, isolation, prediction and analyses)
• explain the theoretical background of these methods, their advantages and limitations as well as possible applications

Skills

• use molecular tools for heterologous expression of genes and proteins
• perform DNA alignments of genes or fragments
• optimize and design primers for PCR amplification of target DNA
• determine variation in microbiome in environmental matrices
• detect and isolate heterologously produced proteins

Type of instruction

• Lectures
• Workshops
• Experimental exercises

Extent and expected workload

150 hours

Exam

Exams