Molecular Biotechnology – Recombinant DNA Technology


Content, progress and pedagogy of the module

Learning objectives


Students who complete the module must be able to

  • account for general technologies in molecular biology and genomic analyses
  • account for basic methods in recombinant DNA technology (cloning, vectors, primer design and transformation)
  • account for methods in genetics, epigenetics, transcriptomics and proteomics
  • account for basic techniques in DNA sequencing (genomes and fragments)
  • explain the basic methods for microbiome assessment and identification
  • explain the methods used in protein engineering (tagging, isolation, prediction and analyses)
  • explain the theoretical background of these methods, their advantages and limitations as well as possible applications


  • use molecular tools for heterologous expression of genes and proteins
  • perform DNA alignments of genes or fragments
  • optimize and design primers for PCR amplification of target DNA
  • determine variation in microbiome in environmental matrices
  • detect and isolate heterologously produced proteins

Type of instruction

  • Lectures
  • Workshops
  • Experimental exercises

Extent and expected workload

150 hours



Name of examMolecular Biotechnology – Recombinant DNA Technology
Type of exam
Written or oral exam
Assessment7-point grading scale
Type of gradingInternal examination
Criteria of assessmentThe criteria of assessment are stated in the Examination Policies and Procedures

Facts about the module

Danish titleMolekylær bioteknologi - rekombinant DNA-teknologi
Module codeK-KT-K1-31
Module typeCourse
Duration1 semester
Language of instructionEnglish
Empty-place SchemeYes
Location of the lectureCampus Esbjerg
Responsible for the module


Study BoardStudy Board of Biotechnology, Chemistry and Environmental Engineering
DepartmentDepartment of Chemistry and Bioscience
FacultyFaculty of Engineering and Science