NMR and MS

2019/2020

Prerequisite/Recommended prerequisite for participation in the module

The module builds on knowledge obtained in the area of Organic and Physical Chemistry

Content, progress and pedagogy of the module

NMR: The physical background for NMR:

  • Nuclear spin, spin in a magnetic field, CW-NMR, FT-NMR, radiofrequency pulses
  • Spectral parameters: chemical shift, scalar and dipolar coupling
  • Spectroscopic technique: 1D experiments with one or more pulses
  • Experimental aspects: construction of NMR spectrometer, experimental NMR, signal treatment, Nuclear Magnetic Relaxation: spin-lattice or spin-spin relaxation and their dependence on molecular mobility, nuclear Overhauser effect
  • 2D-NMR: Meaning of 'chemical shift labeling', magnetization transfer between spins, acquisition of the indirect dimension, homonuclear 2D-NMR (COSY, TOCSY, NOESY), heteronuclear 2D-NMR (HSQC, HMQC)
  • Dynamic NMR Spectroscopy: chemical shift, lineshape analysis, 'coalescence', timescale for NMR
  • Interpretation of NMR spectra: assignment of signals, structure determination of small molecules
  • Selected topics of modern, applied NMR, i.e. NMR of macromolecules, 'magnetic resonance imaging' description of NMR based on quantum mechanics, metabolic profiling via NMR
  • Problems: Interpretation of spectra, identification of compounds based on their spectra, collecting data on the in-house spectrometer, theoretical calculations

MS:

  • History of MS development and applications within biotechnology and chemistry
  • Physical concept behind MS ionization (matrix-assisted laser desorption ionization/electro-spray)
  • Mass analyzer (time-of flight, quadrupol, ion-field)
  • MS/MS sequencing, iondetection, reflectron
  • Application of on-line chromatography (HPLC, GC, CE)
  • Special applications for different MS, i.e. MALDI-TOF-MS and nano-spray followed by MS/MS for analysis of proteins
  • Interpretation of spectra of organic molecules (proteins, peptides and DNA sequences, carbohydrates) and problems to support the theory behind it. 
  • Introduction to mass spectrometry baed bioinformatics

 

Learning objectives

Knowledge

Students who complete the module 

  • Should have knowledge about the theoretical background of NMR and MS, especially about how to get signals and interpretation of signals
  • should have knowledge about the experimental process how NMR and MS data are collected

Skills

  • should be able to interpret 1D and 2D NMR spectra which means to be able to predict a spectrum from a given structure, find an unknown structure based on a given spectrum or be able to assign NMR signals to atoms within a structure
  • should be able to evaluate applications for NMR and MS for chemical/biotechnological/nanotechnological problems
  • should be able to interpret MALDI MS and ESI MS spectra
  • should be able to use correct concept, nomenclature, and symbols from the NMR and MS literature

Exam

Exams

Name of examNMR and MS
Type of exam
Written or oral exam
ECTS5
Assessment7-point grading scale
Type of gradingInternal examination
Criteria of assessmentThe criteria of assessment are stated in the Examination Policies

Facts about the module

Danish titleNMR og MS
Module codeK-BT-B6-14
Module typeCourse
Duration1 semester
SemesterSpring
ECTS5
Language of instructionDanish
Empty-place SchemeYes
Location of the lectureCampus Aalborg
Responsible for the module

Organisation

Study BoardStudy Board of Biotechnology, Chemistry and Environmental Engineering
DepartmentDepartment of Chemistry and Bioscience
FacultyFaculty of Engineering and Science