Forudsætninger/Anbefalede forudsætninger for at deltage i modulet

The module builds on knowledge obtained in the area of Organic and Physical Chemistry.

Modulets indhold, forløb og pædagogik


The physical background for NMR: Nuclear spin, spin in a magnetic field, CW-NMR, FT-NMR, radiofrequency pulses; Spectral parameters: chemical shift, scalar and dipolar coupling; Spectroscopic technique: 1D experiments with one or more pulses; Experimental aspects: construction of NMR spectrometer, experimental NMR, signal treatment, Nuclear Magnetic Relaxation: spin-lattice or spin-spin relaxation and their dependence on  molecular mobility, nuclear Overhauser effect; 2D-NMR: meaning of 'chemical shift labeling', magnetization transfer between spins, acquisition of the indirect dimension, homonuclear 2D-NMR (COSY, TOCSY, NOESY), heteronuclear 2D-NMR (HSQC, HMQC); Dynamic NMR Spectroscopy: chemical shift, lineshape analysis, 'coalescence', timescale for NMR; Interpretation of NMR spectra: assignment of signals, structure determination of small molecules; selected topics of modern, applied NMR, i.e.: NMR of macromolecules, 'magnetic resonance imaging', description of NMR based on quantum mechanics, metabolic profiling via NMR; Problems: Interpretation of spectra, identification of compounds based on their spectra, collecting data on the in-house spectrometer, theoretical calculations.


History of MS development and applications within biotechnology and chemistry, physical concept behind MS ionization (matrix-assisted laser desorption ionization/electro-spray); mass analyzer (time-of flight, quadrupol, ion-field).  MS/MS sequencing, iondetection, reflectron. Application of on-line chromatography (HPLC, GC, CE). Special applications for different MS, i.e. MALDI-TOF-MS and nano-spray followed by MS/MS for analysis of proteins. Interpretation of spectra of organic molecules (proteins, peptides and DNA sequences, carbohydrates) and problems to support the theory behind it. Introduction to mass spectrometry based bioinformatics.



  • should have knowledge about the theoretical background of NMR and MS, especially about how to get signals and interpretation of signals
  • should have knowledge about the experimental process how NMR and MS data are collected


  • should be able to interpret 1D and 2D NMR spectra which means to be able to predict a spectrum from a given structure, find an unknown structure based on a given spectrum or be able to assign NMR signals to atoms within a structure
  • should be able to evaluate applications for NMR and MS for chemical/biotechnological/nanotechnological problems
  • should be able to interpret MALDI MS and ESI MS spectra
  • should be able to use correct concept, nomenclature, and symbols from the NMR and MS literature


Lectures supported by problem solving classes.

Omfang og forventet arbejdsindsats

Kursusmodulets omfang er 5 ECTS svarende til 150 timers studieindsats.



Prøvens navnNMR and MS
Skriftlig eller mundtlig
CensurIntern prøve
VurderingskriterierVurderingskriterierne er angivet i Universitetets eksamensordning

Fakta om modulet

Engelsk titelNMR and MS
Varighed1 semester
UndervisningssprogDansk og engelsk
UndervisningsstedCampus Aalborg


StudienævnStudienævn for Mekanik og Fysik
InstitutInstitut for Materialer og Produktion
FakultetDet Ingeniør- og Naturvidenskabelige Fakultet