NMR and MS


Prerequisite/Recommended prerequisite for participation in the module

The module builds on knowledge obtained in the area of Organic and Physical Chemistry

Content, progress and pedagogy of the module

NMR: The physical background for NMR:

  • Nuclear spin, spin in a magnetic field, CW-NMR, FT-NMR, radiofrequency pulses
  • Spectral parameters: chemical shift, scalar and dipolar coupling
  • Spectroscopic technique: 1D experiments with one or more pulses
  • Experimental aspects: construction of NMR spectrometer, experimental NMR, signal treatment, Nuclear Magnetic Relaxation: spin-lattice or spin-spin relaxation and their dependence on molecular mobility, nuclear Overhauser effect
  • 2D-NMR: Meaning of 'chemical shift labeling', magnetization transfer between spins, acquisition of the indirect dimension, homonuclear 2D-NMR (COSY, TOCSY, NOESY), heteronuclear 2D-NMR (HSQC, HMQC)
  • Dynamic NMR Spectroscopy: chemical shift, lineshape analysis, 'coalescence', timescale for NMR
  • Interpretation of NMR spectra: assignment of signals, structure determination of small molecules
  • Selected topics of modern, applied NMR, i.e. NMR of macromolecules, 'magnetic resonance imaging' description of NMR based on quantum mechanics, metabolic profiling via NMR
  • Problems: Interpretation of spectra, identification of compounds based on their spectra, collecting data on the in-house spectrometer, theoretical calculations


  • History of MS development and applications within biotechnology and chemistry
  • Physical concept behind MS ionization (matrix-assisted laser desorption ionization/electro-spray)
  • Mass analyzer (time-of flight, quadrupol, ion-field)
  • MS/MS sequencing, iondetection, reflectron
  • Application of on-line chromatography (HPLC, GC, CE)
  • Special applications for different MS, i.e. MALDI-TOF-MS and nano-spray followed by MS/MS for analysis of proteins
  • Interpretation of spectra of organic molecules (proteins, peptides and DNA sequences, carbohydrates) and problems to support the theory behind it. 
  • Introduction to mass spectrometry baed bioinformatics


Learning objectives


Students who complete the module 

  • Should have knowledge about the theoretical background of NMR and MS, especially about how to get signals and interpretation of signals
  • should have knowledge about the experimental process how NMR and MS data are collected


  • should be able to interpret 1D and 2D NMR spectra which means to be able to predict a spectrum from a given structure, find an unknown structure based on a given spectrum or be able to assign NMR signals to atoms within a structure
  • should be able to evaluate applications for NMR and MS for chemical/biotechnological/nanotechnological problems
  • should be able to interpret MALDI MS and ESI MS spectra
  • should be able to use correct concept, nomenclature, and symbols from the NMR and MS literature



Name of examNMR and MS
Type of exam
Written or oral exam
Assessment7-point grading scale
Type of gradingInternal examination
Criteria of assessmentAre stated in the Joint Programme Regulations

Facts about the module

Danish titleNMR og MS
Module codeK-BT-B6-14
Module typeCourse
Duration1 semester
Language of instructionDanish
Empty-place SchemeYes
Location of the lectureCampus Aalborg
Responsible for the module


Study BoardStudy Board of Biotechnology, Chemistry and Environmental Engineering
DepartmentDepartment of Chemistry and Bioscience
FacultyFaculty of Engineering and Science