Forudsætninger/Anbefalede forudsætninger for at deltage i modulet

Modulets indhold, forløb og pædagogik

NMR:

The physical background for NMR: Nuclear spin, spin in a magnetic field, CW-NMR, FT-NMR, radiofrequency pulses; Spectral parameters: chemical shift, scalar and dipolar coupling; Spectroscopic technique: 1D experiments with one or more pulses; Experimental aspects: construction of NMR spectrometer, experimental NMR, signal treatment, Nuclear Magnetic Relaxation: spin-lattice or spin-spin relaxation and their dependence on  molecular mobility, nuclear Overhauser effect; 2D-NMR: meaning of 'chemical shift labeling', magnetization transfer between spins, acquisition of the indirect dimension, homonuclear 2D-NMR (COSY, TOCSY, NOESY), heteronuclear 2D-NMR (HSQC, HMQC); Dynamic NMR Spectroscopy: chemical shift, lineshape analysis, 'coalescence', timescale for NMR; Interpretation of NMR spectra: assignment of signals, structure determination of small molecules; selected topics of modern, applied NMR, i.e.: NMR of macromolecules, 'magnetic resonance imaging', description of NMR based on quantum mechanics, metabolic profiling via NMR; Problems: Interpretation of spectra, identification of compounds based on their spectra, collecting data on the in-house spectrometer, theoretical calculations.

MS:

History of MS development and applications within biotechnology and chemistry, physical concept behind MS ionization (matrix-assisted laser desorption ionization/electro-spray); mass analyzer (time-of flight, quadrupol, ion-field).  MS/MS sequencing, iondetection, reflectron. Application of on-line chromatography (HPLC, GC, CE). Special applications for different MS, i.e. MALDI-TOF-MS and nano-spray followed by MS/MS for analysis of proteins. Interpretation of spectra of organic molecules (proteins, peptides and DNA sequences, carbohydrates) and problems to support the theory behind it. Introduction to mass spectrometry based bioinformatics.

Læringsmål

Viden

• should have knowledge about the theoretical background of NMR and MS, especially about how to get signals and interpretation of signals
• should have knowledge about the experimental process how NMR and MS data are collected

Færdigheder

• should be able to interpret 1D and 2D NMR spectra which means to be able to predict a spectrum from a given structure, find an unknown structure based on a given spectrum or be able to assign NMR signals to atoms within a structure
• should be able to evaluate applications for NMR and MS for chemical/biotechnological/nanotechnological problems
• should be able to interpret MALDI MS and ESI MS spectra
• should be able to use correct concept, nomenclature, and symbols from the NMR and MS literature

Undervisningsform

Lectures supported by problem solving classes.

Omfang og forventet arbejdsindsats

Kursusmodulets omfang er 5 ECTS svarende til 150 timers studieindsats.

Eksamen

Prøver